Antibacterial Activity Of Different Plant And Callus Extracts A Comparative Study

Abstract Current study includes antibacterial activity of different plant and callus extract such as Momordica charantia Karela Cucurbita pepo Pumpkin Capsicum annum Chilli Coriander sativum Coriander Brassica nigra Mustard Nigella sativa Black Cumin Trigonella foenum Fenugreek against eight pathogenic bacteria of which five are gram positive such as Staphylococcus aureus Bacillus cereus Bacillus subtilis Streptococcus sp Bacillus megaterium and three are gram negative such as Escherichia coli Proteus vulgaris Pseudomonas aeruginosa. Plant materials were extracted using ethanol. All the extracts showed zone of inhibition against these pathogenic bacteria which were tested by agar well diffusion method. The leaf extract of Momordica charantia Nigella sativa Barassica nigra showed good zone of inhibition as compared to other extracts against S.aureus 13mm 17mm11mm E.coli 13mm10mm18mm P. aeruginosa 11mm12mm10mm Streptocococcus 14mm 15mm 10mm B.subtilis 10mm17mm10mm P. vulgaris 15mm18mm 10mm B.cereus 13mm16mm12mm B.megaterium 12mm15mm10mm. Explants from in vitro grown plants were cultured on MS-Medium with different combination of IAA and Kinetin for callus induction. Among those combination of IAA and Kinetin 0.1 x 0.0 1.0 x 0.0 0.4 x 0.5 1.0 x 0.5 1.5 x 0.5 0.4 x 1.0 0.8 x1.0 0.1 x 1.5 mgL have showed maximum growth of calli.