Modulation Of Fumonisin B1 Toxic Action-Induced By Zeralenone In Human Intestinal Cells Caco-2

Abstract The natural co-occurrence of Fusarium toxins fumonisin B1 FB1 and Zearalenone ZEA in cereal grains is well known. However a few studies have been reported that address to the toxicity of Fusarium toxins mixtures. Thus the aim of the present study was to investigate in the interactive effect of binary Fusarium toxins FB1 and ZEA on intestinal cells line Caco-2 using several cellular endpoints such as caspace-3 activity modulation malonedialdehyde MDA production lactate dehydrogenase LDH leakage as necrosis measure and cells viability as evaluated by lysosome and mitochondria integrities. As results ZEA FB1 led to an antagonistic effect on lysosome and mitochondria damage but the necrosis-induced was more potent when compared to ZEA or FB1 alone showing an additive effect. Moreover MDA production induced by ZEAFB1 was higher than additive effect but not synergistic effect. At last the combined effect of toxins on key apoptosis enzyme caspace-3 kinetic activity was an additive effect after 6 h and 24 h but after 3h ZEA tended to exert its anti-apoptotic action by reducing the enzyme activation by FB1. Taken together the results were contrasted and results suggested that combined effects of binary Fusarium toxins ZEA and FB1 in cell line Caco-2 were unpredictable and varied according to several parameters such as the cellular endpoints and the duration of cells incubation with toxins.