CARBOHYDRATE METABOLISM IN THE SKIN RATS IN INTRADERMAL ADMINISTRATION OF THE PREPARATION OF HIGH-MOLECULAR NATIVE HYALURONAN

At females of white rats of mature age (11-12 months) weighing 280-320 g at interdermal triple introduction of medicine of not stabilized high-molecular hyaluronic acid studied contents in skin in the field of an injection the following indicators of carbohydrate exchange: pyruvate, lactate, activity of a hexakinase, glucose-6- phosphate dehydrogenase, lactate dehydrogenase, levels of a glycogen, glycosaminoglycan totally and hyaluronic acid. The medicine containing 13,5 mg/ml of gel of a gialuronan with a molecular weight of 1 million. It was entered for the 1st, 3rd and 6th day of an experiment by a mesotherapy method. The maintenance of indicators of carbohydrate exchange and activity of enzymes studied for the 2nd, 4th, 7th, 21st and 37th days of experience. Results of a research have allowed to come to conclusion that interdermal course introduction of medicine of high-molecular native hyaluronic acid by method of a mesotherapy brings in skin in an injection zone in the first days an increase in processes of anaerobic oxidation of carbohydrates with mobilization of a glycogen. Ratios a lactate/pyruvate to 118,2%, 152,3% and 146,9%, reduction of maintenance of a glycogen to 90,1%, 76,8% and 78,6% with increase in activity of a lactated ehydrogenеsis to 138,9%, 133,3% and 139,1% respectively testify to it statistically significant increase in skin of content of lactic acid for the 2nd, 4th and 7th days of an experiment to 120,8%, 144,8% and 139,8%. In the remote terms after performing course therapy of medicine of a gialuronan in skin there is an activation of processes of aerobic oxidation to strengthening of use of glucose on the biosynthetic purposes that finds the reflection in reliable increase for the 37th days of an experiment of activity of a hexakinase up to 133,3% and glucose-6-Phosphatdehydrogenesis to 164,1%, decrease in coefficient a lactate/pyruvate to 80,7%, increase in concentration of a gialuronan to 130% and the general glycosaminoglycan to 114,3%. Change in exchange of carbohydrates in skin in the field of interdermal introduction of medicine of highmolecular native hyaluronic acid is reflection of improvement of the exchange processes allowing to prolong esthetic effect of a mesotherapy.