Comparative Analysis Of Heavy-Metal Ion Sensing Mechanisms With Transcription Factors Smtbs From Freshwater Synechococcus Sp. PCC 7942 And Marine Synechococcus Sp. PCC 7002 Evolutionary And Structural Aspects
Journal: International Journal of Scientific & Technology Research (Vol.2, No. 12)Publication Date: 2013-12-15
Authors : Rahul Mahadev Shelake; Kanako Aibara; Hidenori Hayashi; Shunnosuke Abe; Eugene Hayato Morita;
Page : 274-284
Keywords : Index Terms Cyanobacteria; electrophoretic mobility shift assay; heavy metal ion sensing; phylogenetic analysis; SmtBArsR family.;
Abstract
Abstract Metal-responsive transcription factors play a crucial role in the metal ion homeostasis with both cellular metabolism and environmental metal availability. In present work we studied structural and evolutionary features of a transcription repressor SmtB from freshwater Synechococcus sp. PCC 7942 and its homologs from other cyanobacteria in detail. We mined putative SmtB-like zinc sensors from all available sequenced genomes of cyanobacterial species in CyanoBase database. We found marine Synechococcus sp. PCC 7002 consists of similar homologous gene regulation system to that of PCC 7942. In both cyanobacteria the gene expression of SmtA metallothionein is regulated by repressor protein SmtB and they are transcribed divergently. We compared the binding affinities of SmtBs to the operatorpromoter regions of smtA and inhibitory effect of Zn2 with electrophoretic mobility shift assay. From these analyses we found number of complexes between recognition DNA sequences and repressor proteins depends on the structural aspects of DNA sequences of operatorpromoter region. We also found that the 269455 sites are completely identical in these two proteins but differences in 269453N sites andor operatorpromoter region might be responsible for dissociation of protein-DNA complexes at different zinc ion concentrations 16 uM of Zn2 in PCC 7942 and 256 uM of Zn2 in case of PCC 7002.
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