Evaluation Of Toxicological Effects Of Carbon Tetrachloride On Liver Enzymes Of Wistar Rats

ABSTRACT This work is aimed at determining the effects of carbon tetrachloride on liver enzymes of adult wistar rats following oral administration. Twenty adult wistar rats weighing between 190-220kg were used. They were allocated into four groups ABC 26D of five animals each. Group A animals served as the control and received 0.5ml of distilled water. The experimental groups BC 26 D received 0.1ml 0.2ml and 0.3ml of carbon tetrachloride respectively for 21 days. Twenty four hours after the last administration the animals were weighed anaesthetized under chloroform vapour and dissected. The liver tissues were removed and weighed. Blood samples were collected by cardiac puncture using sterile syringes and needles. Blood for serum preparation was collected into sterile plain tubes without anti-coagulant. Serum samples were separated into sterile plain tubes and stored in the refrigerator for analysis. The activities of serum aspartate aminotransferase AST alanine aminotransferase ALT and alkaline phosphatase ALP were determined using randox kit method. The final body weight of the experimental groups were significantly lower P0.001 than the control A. the levels of mean aspartate aminotransferase alatine aminotransferase and alkaline phosphatase in group D were significantly higher P0.001 than groups BC and control A. The levels of mean aspartate aminotransferase alanine aminotransferase and alkaline phosphotase in group C were significantly higher P0.001 than group B and control A. The result from this study showed that adverse biochemical changes are associated with animals in groups BC and D. The present study indicated dose-dependent on the liver enzymes of rats administered with CCL4. Our findings therefore suggest that chronic CCL4 consumption may put the liver at risk of adverse biochemical alterations and histopathological lesions.