In Vitro Efficacy Of Lactic Acid Bacteria With Antifungal Activity Against Fusarium Sp. CID124-CS Isolate From Chilli Seeds

Lactic acid bacteria LAB are known as to have inhibitory activity against fungi and other pathogens. In this study LAB from soil and fermented chilli fruits were evaluated for their inhibitory activity against Fusarium sp. CID124-CS that was isolated from chilli fruits. Three LAB isolates Lb. plantarum1-MSS P. pentoceous1-MSS isolated from soil one Lb. plantarum1-FCF from fermented chilli and two from ATCC culture Lb. acidophilus ATTCC314 and Lb. plantarum ATCC8014 showed strong inhibitory activity against growth of target fungi evaluated by well diffusion assay showed high growth inhibition 6.05 mm to 7.60 mm within 48 h at 28oC. Adding LAB supernatant to Potato Dextrose Broth PDA with fungi reduced mycelia growth from 36.00 to 60.00. Similarly fungal mass reduction with cells of LAB in De Man Rogosa and Sharpe Broth MRSB ranged 98.0 to 99.9 after 72 h incubation at 28oC by micro tire plate assay. Whereas conidial germination in MEB with LAB supernatants were reduced by 93.3 to 96.6 using micro titre plate assay. This study showed that cells and CFS of LAB isolated from soil have antifungal activity and could be used as antifungal agent against Fusarium sp. CID124-CS that infect to chilli.