Optimization of Process Parameters for Xylanase Production by Bacillus atropheaus [KJ 590121] SD9 Isolated from Sludge using Response Surface Methodology

In recent year, xylanase has become an essential option for eco-friendly industrial biotechnological applications and there is a rising demand for large scale production. In this study, Bacillus atropheaus SD9 isolated from sludge was tested for the xylanase production under submerged cultivation conditions. Maximum xylanase activities were achieved by optimizing process conditions i.e. temperature, pH, inoculums size and substrate concentration that significantly affect the xylanase production. Central Composite Design (CCD) quadratic response surface was applied to explicate these four factors. Statistical analysis of the results showed that optimized parameters had a significant effect on xylanase production. These optimization conditions resulted in a 3-fold increased level of the xylanase (85.16 U/g) production after 120 h of fermentation; whereas the value predicted by the quadratic model was 80.07 U/g/ml. High xylanase activity may broaden the prospectus of industrial scale. This statistical approach has been proved a powerful tool for the development of cultural conditions and to gather sufficient information with minimum number of trials for enhanced production of xylanase from this strain.