Safe and Efficient siRNA Therapy using Nanosecond Laser and Electrical Pulses

RNA interference (RNAi) is a natural phenomenon existing in plants, insects, and many mammals, involved in gene silencing or the inhibition of gene expression. Small interfering RNAs (siRNAs) could be used as potential tools to implement this process that could lead to treatments in diseases, such as cancer. However, the delivery of siRNA to eukaryotic cells is a challenge due to its lower cellular uptake and easy degradation by enzymes in blood, tissues, and cells. Nanosecond duration laser and electrical pulses-based siRNA delivery is an upcoming therapeutic tool to deliver various siRNAs, both in vitro and in vivo.