Indicators of Metabolic Endotoxemia in Ovarietomated Rats with Metabolic Syndrome during Pharmacological Correction

Nowadays the problem of metabolic endotoxemia is widely discussed in scientific literature. Connection between hormonal and particularly estrogen status and metabolic endotoxemia had been scientifically proved. However, there is no sufficient justification of the immunotropic compounds and exogenous estrogen influence on the state of Endotoxemia during postovariaectomia metabolic syndrome. In the laboratory of hormone-like compounds synthesis of the State Institution “V. Danilevsky Institute for Endocrine Pathology Problems of National Academy of Medical Science of Ukraine” (Kharkov, Ukraine) the derivative of levamisol-308 (PL-308) which corresponds to the class 5 of toxicity (almost non-toxic compounds) according to the acute toxicity data had been synthetically produced. The purpose of the research was to determine the state of metabolic endotoxemia in ovariectomized rats during metabolic syndrome (MS) for liposaccharide (LPS) antibodies and introducing compounds with immunomodulatory properties of levamisole and its derivative PL-308 and exogenous 17β-estradiol. Materials and Methods. The tests were conducted on the 3-month white female rats of the Vistar line. We reproduced the hypoestrogenia by the way of two-side overiaectomia with the light etherization. We preceded the induction of MS within 2 weeks after the operative intervention. The high-carbohydrate diet (HCD) was provided by the free access to the 30% sweet water as a potable water during 5 and 8 weeks. Levamisole and its derivative PL-308 were provided for 5 weeks by the means of a zonde. 17β-estradiol was provided intramuscularly once daily for two days (10 μg / kg body weight) for 8 weeks. The state of metabolic endotoxemia was assessed by the level of antibodies to the endotoxin, which were determined by ELISA method. Results. Hypoestrogenia is an independent reason of antibody level increase of LPS. The combination of estrogen deficit with the MS didn`t influence the endotoxemia level. The compounds with immunomodulation capacities as levamisole and its derivative PL-308 have decreased the antibody level to LPS with the ovariaectomia rats with MS to the level of intact animals, when 17β-estradiol has decreased but not normalized the endotoxin level with the similar group of rats. Conclusions. The normalization of LPS level which was observed only during levamisole usage and its derivative PL-308, testifies that the immune correction is an important step in women rehabilitation with the metabolic misbalance and is crucial to the metabolic endotoxemia decrease along with the protective treatment of the immune deficit conditions.