Examination of the Kinetics of Antimicrobial Substance Release from Polymer Compounds by Using Microbiological Method in Conjunction with Mathematical Analysis

Antimicrobial agents that have recently been effective in the wound infections treatment are rapidly losing their effectiveness due to the development of microorganisms' resistance. New polymeric materials constantly appear, promising the creation of systems for the local delivery of chemotherapeutic agents. At the stage of such compounds' preclinical study, the question on the determination of patterns of antimicrobial release in the environment arises. Within current study, the microbiological "wells" method was used to examine antimicrobial excretion, accompanied with mathematical analysis using Microsoft Excel. The pharmacokinetic properties of new compounds can be numerically described and statistically compared by means of this method. Materials and methods. Seven samples of a polymer antimicrobial compounds were made, which presented a mixture of polymethyl methacrylate (PMMA) or zinc phosphate cement, biocomposite Synthe-Bone and decamethoxin in different ratios. Each of the created compounds was distinguished by the main factors influencing the substances' release from the polymeric systems of local drugs delivery, like: decamethoxin concentration, the adsorption capacity of the matrix base and its biodegradability in water. Microbiological method of "wells" accompanied with mathematical analysis conducted by means of Microsoft Excel was used to describe the antimicrobial excretion. Results. The antimicrobial activity of each sample was studied in triplicate repetition by the inhibition zone around the "well", which is directly dependent on the decamethoxin concentration in the solution. By inhibition zones around the control wells, where decametoxin solution was put at known concentrations, a calibration scale and a conversion formula were constructed. This formula allows us numerically describe the pharmacokinetic properties of new compounds and carry out their statistical comparison. The kinetics of decamethoxin release from certain samples has been described by a polynominal equation that allows to predict the duration of the antibacterial effect of the compounds and to calculate the amount of the drug released at established intervals. Conclusions. Using this method, the kinetics of antimicrobial substance release is described through mathematical equations, which allows predicting the duration of antimicrobial efficacy and determining the amount of the released substance. The equations and graphs of decamethoxin excretion reflect patterns that depend on the physico-chemical properties of the constituents in the polymer compounds.