MITOCHONDRIAL RESPIRATION AND GLYCOLYSIS IN THE BRAIN HOMOGENATE UNDER CONDITIONS OF PARTIAL ISCHEMIA

Background. Estimation of changes in neuronal bioenergetics in the brain its partial ischemia (BPI) is crucial for finding new ways of correcting brain ischemic lesions. The aim of the study was to investigate the changes both in mitochondrial respiration (MR) and processes of anaerobic glycolisis in the rats` homogenate of the brain in terms of partial ischemia. Material and methods. In an experiment on 19 white female rats we studied the dynamics of changes MR of the brain after 1 hour and 3 days of BPI by means of the polarographic method using succinate or L-malate/L-glutamate as well as ADP. The activity of anaerobic glycolysis was assessed with respect to the changes in the concentration of lactate and pyruvate in plasma and homogenate of the brain. Results. We identified inhibition of the mitochondrial respiration in rat brain homogenate as well as increased level of lactate in cerebral homogenate, which was more pronounced in plasma after 1 hour of BPI compared to 3 days of BPI which was more pronounced in cerebral homogenate. The level of pyruvate was more pronounced in plasma after 1 hour of BPI compared to 3 days of BPI only. Conclusion. Partial cerebral ischemia of the rat due to the ligation of the right common carotid artery leads to a remarkable reduction in the respiratory activity of the brain's mitochondria and to the dissociation of oxidation and phosphorylation in the mitochondria, which in turn aggravates the neuronal energy deficit induced by the disturbance of the supply of oxygen by blood. The search for effective methods for correcting energy processes in the mitochondria of the brain is one of the key directions in the pathogenetic therapy of cerebral ischemia. Partial cerebral ischemia can be of interest to researchers for use as a model for screening the means of pharmacological correction of mitochondrial respiration disorders in brain cells during its ischemia.