IMMUNOHISTOCHEMICAL LOCALIZATION OF CHOLECYSTOKININ AND HISTAMINE IN GASTROINTESTINAL TRACT IN FLOWER FISH

Abstract: In this study, our aim was to determine the distribution of specific immunoreactivities against the antisera Cholecystokinin (CCK) and Histamine in some regions of the digestive tract of the flower fish (Pseudophoxinus antalyae). The samples were taken from the enlarged area after oesophagus and anterior, middle and posterior part of intestine, and the Avidin-Biotin-Immunoperoxidase method was applied. Histamine and Cholecystokinin immunoreactive cells were found to be more frequent in enlarged region after oesophagus than in gut. There immunoreactive cells were distributed among surface epithelium, epithelium between surface crypt, and crypts proper. Despite determination of Histamine immunoreactivity in enlarged region after oesophagus, connective tissue of intestine and ganglion cells, CCK immunoreactivity was not be detected in the same regions and cells. Key words: Pseudophoxinus antalyae, gastrointestinal tract, endocrine cells, immunohistochemistry