THE DEVELOPMENT OF PCR SYSTEM FOR IDENTIFICATION OF BEET NECROTIC YELLOW VEIN VIRUS AND MONITORING IN UKRAINE

The ELISA screening of beet necrotic yellow vein virus (BNYVV) in agrocenoses of Ukraine (Western and Central Ukraine) was performed and the presence of BNYVV was shown in samples of roots of sugar beet plants selected in Chemerovetsky region of Khmelnitsky oblast. The bioinformative analysis of nucleotide sequences of isolates of BNYVV deposited in genetic databank was made. The conserved gene sequence encoding coat protein was established and specific primers for nucleotide sequences of the genome of the BNYVV were developed. The specific oligonucleotide primers were synthesized. The laboratory testing of synthesized primers and the optimization of polymerase chain reaction were made. The optimum temperature of primers hybridization was 54 °C - 62 °C and the highest production of amplicons was registered by the concentration of Mg+2 was 2,5 mM