ANTIMICROBIAL ACTIVITY OF SOME POTENTIAL GREEN ALGAL STRAINS ISOLATED FROM BUNDELKHAND REGION UTTAR PRADESH

To study antimicrobial activity of some potential green algal strains viz., Scenedesmus abundans, Nannochloropsis oculata and Spirogyra condensata, against four human bacterial pethogens. Methods The characterization of the antimicrobial activity was used disc diffusion method against four human pathogenic bacteria viz., Escherichia coli, Staphylococcus aereus, Bacillus cereus and Pseudomonas aerugenosa. Streptomycin disc were used as a positive control and only solvent were used as a negative control. Results The methanolic extract of Scenedesmus abundans, Nannochloropsis oculata, and Spirogyra condensata showed the antibacterial activity against four pathogenic bacteria viz., Escherichia coli, Staphylococcus aereus, Bacillus cereus and Pseudomonas aerugenosa with the inhibition zone( 15, 16, 22, 17 mm) (15,15,26,17mm) and (25, 16, 26, 20mm) respectively. The ethanolic extract of Scenedesmus abundans, Nannochloropsis oculata, and Spirogyra condensata showed the antibacterial activity against four pathogens viz, Escherichia coli, Staphylococcus aereus, Bacillus cereus and Pseudomonas aerugenosa with the inhibition zone (20, 23, 23,21 mm) (19, 25,33, 24 mm) and (24,23,32,22 mm) respectively. The acetone extract of Scenedesmus abundans, Nannochloropsis oculata, and Spirogyra condensata showed the antibacterial activity against four pathogens viz., Escherichia coli, Staphylococcus aereus, Bacillus cereus and Pseudomonas aerugenosa, with the inhibition zone (18, 34, 23, 20 mm) (00, 17, 28, 12 mm) and (17,13,15,15) respectively. The di ethyl ether extract of Scenedesmus abundans, Nannochloropsis oculata, and Spirogyra condensata showed the antibacterial activity against four pathogens viz., Escherichia coli, Staphylococcus aereus, Bacillus cereus and Pseudomonas aerugenosa with the inhibition zone (18, 29, 17, 15 mm) (00, 10, 12, 15 mm) and (00,15,8,9 mm) respectively. Streptomycin disc showed the antibacterial activity againt four pathogens viz., Escherichia coli, Staphylococcus aereus, Bacillus cereus and Pseudomonas aerugenosa with the inhibition zones of 28, 15, and 22 mm respectively. Conclusions The present studies conclude that acetone extract of Scenedesmus abundans showed the maximum antibacterial activity against Staphylococcus aereus with the inhibition zone 34 mm. The ethanolic extract of Nannochloropsis oculata showed the maximum antibacterial activity against Bacillus cereus with the inhibition zone 33 mm. The ethanolic extract of Spirogyra condensata showed the maximum antibacterial activity against Bacillus cereus with the inhibition zone 32 mm with reference to antibiotic disc Streptomycin showed antibacterial activity against Staphylococcus aereus with the inhibition zone 15 mm where as antibacterial activity against Bacillus cereus with the inhibition zone 28 mm. Further phytochemical studies are needed to elucidate the components responsible for antibacterial activity of these extract against bacteria.