Development and Validation of Bisacodyl Analysis Method in Tablet with Absorbance Method and Area under Curves Method in Ultraviolet Spectrophotometry

A precise, accurate, fast, and economical simple method has been developed and validated for the analysis of bisacodyl tablets using ultraviolet spectrophotometry with absorbance method and area under the curve. This method is carried out at a maximum absorption wavelength of 263.60 nm and the area under the curve in the range 244.40 - 297.60 nm using a 0.1 N hydrochloric acid solvent. The method of analysis is validated by linearity, detection limits, quantification limits, precision, and accuracy. The linearity of the absorbance method shows the regression equation y = 0.02156x + 0.09258 (r = 0.99963), and the linearity of the wide area method under the curve shows the regression equation y = 0.30618x + 1.17400 (r = 0.99787). Limit detection and quantification limits on the absorbance method 1.411099 and 4.276059, respectively, while the detection and quantification limits on the method of area under the curve 1.968774 and 5.9659197, respectively. Accuracy obtained by the absorbance method and the area under the curve also meet the requirements in the validation of 80 - 120%. The precision obtained by the absorbance method and the area under the curve fulfilled the validated requirement with a yield of RSD ≤ 2%. Statistical analysis was performed by paired sample t test which showed that the two methods did not significantly.