ELUCIDATION OF BIOFILM INHIBITION IN DIFFERENT CLINICAL ISOLATES USING NATURALLY ISOLATED COMPOUNDS FROM ALBIZIA ODORATISSIMA

This study was planned to investigate phytochemical constituents were isolated from the plant Albizia odoratissima and its antibiofilm activity were analysed. Three flavone compounds namely Eupatorin (1),6-methoxy flavone (2),6,2',4'-trimethoxy flavone(3) isolated from the stem bark and structure of the compounds were established based on NMR prediction. Their were no biofilm results in all the three compounds.The antibiofilm activity was evaluated against three reference strains Pseudomonas aeruginosa,Klebseilla pneumonia, Staphylococcus epidermidis by microtitre plate method. Using above reference strains MIC and biofilm inhibition work were done by quantification and SEM. SEM images of the species biofilm inhibition confirmed compound 1,2 and 3 inhibits P.aeruginosa biofilm formation by 93.93%,95.04% and 94.72% at a concentration of 1ng/ml,1ng/ml and 1mg/ml. To compare the results compound 2 showed that greater biofilm inhibition activity against P.aeruginosa at very low concentration of (1ng/ml). The biofilm inhibition of P.aeruginosa of compound 2 on urinary catheter was evaluated using scanning electron microscopic analysis. Key words: Isolated flavones ∙ Antibiofilm ∙ Bacteria ∙ SEM ∙ Catheter