Potentiation of antifibrotic activity of Atacand using grape seed extract (Gervital) in male albino rats

Carbon tetrachloride (CCl4) is a known potent hepatotoxic agent. The present investigation clarified the ameliorative effects of Atacand and Gervital on CCl hepatotoxicity. 24 male albino rats were divided into 4 groups, 6 rats each. Group I, served as normal control. Group II, animals were injected subcutaneously with CCl (1ml/kg b.w.) twice a week for 90 days. Group III, rats were injected with CCl4, as Group II, then treated orally with Atacand (8mg/kg b.w.), daily, for 30 days. rats were injected with the same dose of CCl then treated with Atacand (8mg/kg b.w.) along with Gervital (100mg/kg b.w.), orally, daily for 30 days. treatment showed a marked deterioration of liver function as a high increase in the levels of serum AST and ALT (153.8% and 157.8%, respectively). Also a significant decrease in liver antioxidant machinery was represented by reduced glutathione (GSH, 68.1%), glutathione transferase (GST, 69.9%) and glutathione reductase (GR, 65.65%). On the other side, the levels of liver protein carbonyl groups (PCO) and lipid peroxidation (LPO) were elevated 439.6% and 258.7%, respectively, compared to their control groups. These results indicated th damage as a result of CCl4-induced liver fibrosis. Both treatments with Atacand or Atacand + Gervital showed significant ameliorative effect for both liver and serum CCl4-induced alterations. The current study recorded the treatment with Gervital along with Atacand antifibrotic effectiveness than that of Atacand Gervital acted as a potentiator for antifibrotic activity of Atacand.