THE STUDY OF ANTIOXIDANT ACTIVITY OF BLACK GRAPES EXTRACT TO PREVENT DNA DAMAGE

Introduction: The side products of cellule metabolism like ORSs can damage DNA alkalis and cause restrain in simulation. To avoid any kind of damages and disturbances in cellules they should be controlled and eliminated. Active kinds which are produced in stress oxidation can cause damages to DNA and so they are mutagenic which can prevent cellule dying, increase in reproduction, invasion and metastasis. Different fruits' juices especially pomegranate juice is studied as anti-oxide synthesis that cause better anti-oxide condition and prevent DNA break. Yet black grape juice is a natural one that contains the most beneficent group of flavonoids and pro anti cyanide oligomers. Grape (vitis vinifera) is one of the most different fruits that are used fresh or processed. The stone and peel of black grape contains active compounds specially flavonoid, anthocyanidin, pro anthocyanidin, pro cyanidin and resveratrol that are strong eliminators for free radical. Because of the importance of genome protection, in this study the protective effect of black grape juice as a resource of anti-oxidant in controlling the DNA break is discussed. Materials and Methods: pBR322 plasmid and a nucleotide sequence which is reproduced from ITGB3 gene has been chosen as a model of PCR output. Some kinds of DNA were exposed to different density of black grape juice. Results: According to results of this study, the best concenteration of black grapes juice for prevention of DNA damage of genomic DNA and plasmid pBR322 were 3.100 and 1.100 respectively. Conclusion: The results indicatedthat the antioxidant activity of black grapes juice can inhibit the production of free radicals and DNA damage. Key words: DNA damage, Fenton reaction, black grape juice, ITGB3 gene, Antioxidant activity, Free radical