Comparison of different populations of fennel (Foeniculum vulgare Miller.) in terms of chemical compounds in the callus induced from tissue culture

Abstract Fennel (Foeniculum vulgare Miller.) herbaceous, perennial aromatic Apiaceae family is valuable secondary metabolites that are widely used in the food industry. This medicinal plant in the treatment of oral flatulence, indigestion and muscle spasms, as well as enhancing milk is used as a topical antimicrobial used. Seed Fennel After sterilization, the Petri dishes containing MS medium without growth regulators on the bridge of the paper medium, and the temperature 24 ° C with 16 hours light and 8 hours dark placed, after 15 days, the plant height of about 10 cm was the nodes first (hypocotyls) are used as explants. Induction of callus and explants cultured on MS medium with hormonal treatments 2,4-D +Kinetin and NAA+ BAP Factorial based on completely randomized design with six treatments in each population were used and callus weight was measured. In order to identify the compounds present in the extracts from the callus were used method GC/MS. Lorestan population with the highest limonene and Khroslari population with the highest alpha-pinene in the MS (1mg l-1 2,4-D +1mg l-1 Kin) with of hormonal treatments showed.