The effect of different germination media on pollen germination in vitro of the walnut (Juglans regia L.) cultivar ‘Ibar’

Abstract Different culture media were tested in an attempt to improve the knowledge of the most suitable germination media for studying in vitro pollen germination capacity of the Serbian walnut cultivar ‘Ibar'. The research was conducted on agar media, as a four-factorial experiment, with different concentrations of agar (0.6%, 0.8% and 1%), sucrose (10%, 15% and 20%), H3BO3 (0 ppm, 200 ppm and 400 ppm) and CaCl2 (0 and 50 ppm). Pollen germination was maximized (38.5%) when the germination medium contained 0.6% of agar, 15% of sucrose, 200 ppm of H3BO3 and 50 ppm of CaCl2. Significant differences in pollen germination were observed in response to changing concentrations of agar, sucrose, boric acid and calcium chloride. Significant interactions were detected between most of these substances.