EVALUATION OF DIFFERENT ROUTES OF VACCINATION BY CLONE VACCINE ON HUMORAL ANTIBODY RESPONSE

Prevention of Newcastle disease in broiler birds is a priority for successful poultry industry. The present study evaluates the immune response to Clone 30 live vaccine alone and in combination with inactivated vaccine administrated by different routes in broiler chickens. To evaluate the various route of vaccination and inactivated vaccines on antibody response, Clone vaccine was administered in different routes such as eye drop, spray, and drinking water with or without inactivated vaccine. Hemagglutination inhibition (HI) and enzyme-linked immunosorbent assay (ELISA) methods was used to assess the antibody response. Results indicated significant difference among different groups and the antibody titer showed highest in eye drop with inactivated vaccine group in both the tests at 42 th. day.