PROLACTIN GENE POLYMORPHISM IN HARINGHATA BLACK CHICKEN BREED

PCR-SSCP analysis was carried out to investigate the polymorphism of prolactin gene in Haringhata Black chicken. The amplified fragment (497 bp) of promoter region of prolactin gene revealed three types of SSCP banding pattern arbitrarily assigned as CC, CD and DD genotypes on 10 percent polyacrylamide gel. The heterozygous CD genotypes showed presence of maximum five bands while rest two homozygous genotypes CC and DD showed three and two bands, respectively. In the present study, genotype frequencies were found to be 0.622 for CC, 0.341 for CD and 0.037 for DD genotype with respect to promoter region of prolactin gene. The frequencies of C and D alleles were estimated as 0.793 and 0.207, respectively. The calculated Chi square value revealed that the population was in Hardy Weinberg Equilibrium with respect to promoter region of prolactin gene in Haringhata Black Chicken.