EVALUATION OF α- AMYLASE INHIBITION ASSAY IN VARIOUS FOLIAR EXTRACT OF SARACA ASOCA (ROXB.)

Diabetes is a metabolic illness, caused by the deficiency in production of insulin or ineffectiveness of the insulin. According to the report of WHO on diabetes indicate that in 2014, 8.5% of adults aged 18 years and more than 18 years had diabetes and in 2012 approximately 105 million death were caused by diabetes. A theoretical approach to reduce the risk of diabetes is reduction in the function of carbohydrates hydrolyzing enzymes such as α-amylase. α- amylase enzyme is major digestive enzyme and involves in the breakdown of long chain of carbohydrates. It is a protein enzyme that hydrolyses α bond of large, α- linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. In this study foliar extracts of Saraca asoca were used. For preparation of foliar extract, three different solvents such as petroleum ether, ethanol and distilled water were used. Saraca asoca oftenly used in all parts of India as medicine in the treatment of various diseases. Results of this study showed significant inhibition of alpha amylase at all the selected concentration. Distilled water extract showed inhibition of 55.84%, 44.86% and 38.55% respectively, in case of ethanol the reduction was 54.15%, 46.42% and 43.39% respectively whereas in petroleum ether it was 52.85%, 46.42%, 31.95% respectively. Keywords: Diabetes, Foliar extract, DNSA method, Saraca asoca, α- amylase.