Mass Culturing of Stem and Bulb Nematode (Ditylenchus dipsaci) for use in screenİng and İmpression Training on Carrot Discs

Stem and bulb nematode, Ditylenchus dipsaci, is a very important plant parasitic nematode, has a very wide host range and causes economic yield losses in many cultural plants in worldwide. Its races are very diverse and found in most temperate areas of the world. D. dipsaci is a migratory endoparasite, has the ability to enter into a dormancy stage. Genetic resistance offers one of the best control methods within the integrated pest management strategies for D. dipsaci. However mass rearing of stem and bulb nematode requires for the resistant studies including screening for this nematode species. The aim of study is focused on alternative rearing methods using carrot discs as a food source, culturing medium and provide a clearly outlined and visually informative guide. Carrot discs enable the rearing of high numbers of individuals of D. dipsaci for timely use in experiments and for screening purposes in under sterile conditions to provide a clean, same and pure source of inoculum. The carrot disc method has been shown to be suitable for stem and bulb nematode multiplication.