Oocyte Retrieval Methods, Grade and Percentage of Oocytes in Goats

The present study was undertaken to assess the relative efficiency of three different collection techniques of percentage and grades of oocytes in goats of Hyderabad. The mean oocyte recovery from aspiration technique, 2.15 ± 0.31 and 2.9 ± 0.46 in dissection technique and 6.55 ± 0.57 and 4.51 ± 0.46 in slicing technique. The mean oocyte recovery rate was 1.5 ± 0.22 and 1.0 ± 0.33 under aspiration technique, 2.58 ± 0.47 and 2.43 ± 0.36 under dissection technique and 5.26 ± 0.46 and 6.3 ± 0.71. The mean numbers of oocytes of different grades recovered were 0.34 ± 0.03, 0.62 ± 0.07 and 1.37 ± 0.09 in aspiration, dissection and slicing techniques, respectively. Among the different grades of oocytes, significantly higher percent of ‘A' (27.78) and ‘B' (38.89) grade oocytes were retrieved by aspiration and dissection technique when compared to slicing technique. With respect to ‘C' and ‘D' grade oocytes, significantly higher numbers of oocytes were retrieved by slicing technique than the aspiration and dissection techniques. Out of 437 oocytes retrieved in the present study, significantly higher percent (50.57) yield was by slicing technique, followed by dissection technique (37.07) and aspiration (12.53).