Evaluation of Antitumor Properties of Methanol Extract Of Green Tea against Daltons Ascites Lymphoma Cells

In the present investigation the efficacy of Methanol Extract of Green Tea (MEGT) on the antioxidant and histological parameters of DAL cell lines was evaluated. The antioxidant assay was performed with liver tissue and evaluation was carried out by measuring the level of lipid peroxidation (LPO) and the amount of enzymatic (SOD, Catalase, GSH and GPx) antioxidant systems. In DAL bearing mice the level of LPO was significantly elevated. Treatment with MEGT was found to reduce the level near to normal values.This reflects the decrease in free radical production and the subsequent reduction in oxidative stress. Similarly MEGT at different doses increased the Catalase levels in a dose dependent manner, which along with the restoration of LPO and GSH content to near normal levels indicates the antioxidant property of MEGT. Histological examination of the liver tissue under the light microscope showed the effects of MEGT on the structural integrity of the cells. The liver of normal animals showed normal histological appearance with normal architecture, whereas in DAL control mice, a cluster of atypical cells with dark nucleus were observed in the hepatic parenchyma. The animals treated with MEGT exhibited almost normal histological appearance of liver as in the case of normal control mice showing the hepatoprotective activity of green tea against DAL cells.