An Immunochromatography Assay for Rapid Diagnosis of Brucella Infection in Cattle

Brucellosis is a globally widespread bacterial disease which affects ruminants and other animal species. The main causative agents are Brucella abortus and Brucella melitensis. Investigations were carried out on 200 cattle, from different farms and mobile flocks suspected of suffering from brucellosis from different localities in Bharatpur district (Rajasthan) for evaluation of the sensitivity and specificity of immunochromatographic Assay (ICA), with commonly used serological test, i.e., RBPT. Out of this 200 sample 42 cattle were found positive for Brucella infection using Rapid B. Brucella Ab Kit and same samples were subjected for RBPT which shows 39 (19.5%) samples were seropositive for Brucella infection. It can be concluded that this assay could be ideal as a field test for developing countries and rural settings, suitable for large-scale screening or presumptive test.