Molecular Charcterization of Infectious Bronchitis Virus from Eastern India

Forty eight samples of trachea, lung, kidney, liver, spleen, heart, intestine, proventriculus, gizzard, pancreas samples were collected from gout affected birds or received from commericial poultry farms located in the states of Odhisa, Chhattisgarh, West Bengal, Karnataka, The paraffin embedded tissue were cut into 4 to 6 μm thickness and stained with haematoxylin and eosin. The tissue sections were also subjected to De Galantha's stain to rule out urate crystals. Clinically, the birds were remained with severe respiratory symptoms. Polymerase chain Reaction, sequence and phylogenetic analysis were performed. Microscopic examination revealed sinusoid distension, vacuolar degeneration and necrosis of hepatocytes. Kidney revealed tubular epithelial degeneration and necrosis with tophi formation as urate deposit. Out of 48 samples subjected by RT-PCR assay, one sample revealed amplification at 240 bp of Infectious bronchitis virus. Among the 48 samples suspected for gout, (Odisha N-18, Tamil Nadu N-14, West Bengal N-8, Karnataka N-8), one sample from West Bengal showed positivity for IBV (12.5%) other 47 samples were found negative.