Comparative Efficacy of Various Antigens from Setaria Sp. in the Serodiagnosis of Setarial Microfilariosis in Bovines Using Counter Current Immuno-electrophoresis

Counter current immunoelectrophoresis was used in the serodiagnosis of microfilariosis caused by Setaria spp. in bovines. Three antigens viz., whole worm antigen, excretory secretory antigen and microfilarial antigen were prepared from Setaria worms and used in the test. Whole worm antigen was prepared from the homogenate of adult female Setaria worms. Excretory secretory antigen was prepared by in vitro cultivation of adult gravid female Setaria worms in Dulbecco's Modified Eagles medium and microfilarial antigen was prepared by incubating adult gravid female Setaria worms in Tyrode solution. Results revealed that microfilarial antigen was useful in the serodiagnosis of microfilariosis caused by Setaria compared to whole worm antigen and excretory secretory antigen.