ResearchBib Share Your Research, Maximize Your Social Impacts
Sign for Notice Everyday Sign up >> Login

Simple ELISA using Polyclonal Antibody and Lectin for Determination of Soluble Chitosan - Like Polysaccharide in Bacterial Culture

Journal: International Journal of BioAnalytical Methods & BioEquivalence Studies (IJBMBS) (Vol.04, No. 01)

Publication Date:

Authors : ;

Page : 76-81

Keywords : ELISA; Chitosan; Biopolymer; Polysaccharides; Lectin; Citrobacter.;

Source : Downloadexternal Find it from : Google Scholarexternal


We have developed a simple enzyme-linked immunosorbent assay (ELISA) for the direct determination of chitosan-like polysaccharide (CP) produced in the cultures of Citrobacter strains. CP in sample solutions was reacted with anti-CP rabbit polyclonal antibody immobilized in a 96-well microtiter plate and sandwiched with horseradish peroxidase-conjugated lectin, followed by color development by the enzyme activities. Using standard chitosan solutions, a good standard curve (R2=0.977) was established between 0 and 250 μg l-1 with the average coefficient of variation of 7.0%, and CP in the diluted culture supernatants could be directly determined. This method can apply for the direct determination of other polysaccharides in solution by selecting an inexpensive custom-made polyclonal antibody and an appropriate lectin, both of which may be commercially available.

Last modified: 2018-01-30 15:52:04