TEST OF ANTIMICROBIAL ACTIVITY OF TANNINS EXTRACT FROM GUAVA LEAVES TO PATHOGENS MICROBIAL

This study aims to determine the antimicrobial activity of tannin extracts from guava leaves against pathogens microbial. The method used for qualitative analysis with the tannins are formed by the intensity of the color is blackish green FeCl3 compounds. In the quantitative analysis of tannins used variations of organic solvent (ethanol with a concentration of 30 % , 50 % , 70 % ). Levels of tannins in the sample solution were calculated with Tannates Acid Equivalent (EAT). Test of antimicrobial activity using diffusion agar method. The results showed levels of tannins in leaves of guava with ethanol 30 %, the which is 2.351 mg /g , ethanol 50 % is 1.728 mg g , ethanol 70 % is 1.835 mg / g . The best solvent to extract the highest levels of tannins with ethanol 30 % by value of tannin levels 2.351mg /g. Tannin inhibitory activity on five different pathogens microbial. This is because the composition of the cell wall of the microbe fifth different. The results showed that the extracts rich in tannins can inhibit the growth of Escherichia coli, Pseudomonas aureginosa, Staphylococcus aureus, Aspergillus niger and Candida albican .