Evaluation of Antimicrobial Efficacy of Lyophilized Platelet Lysate against Organisms Isolated from Infected Root Canals: An in vitro Study

INTRODUCTION: The technically demanding endodontic treatment sometimes faces failures because of various polymicrobial infections. Common organisms isolated from such infections are Enterococcus faecalis and Candida albicans. Several researches were made with various materials to eradicate such organisms. The study material used here is Lyophilized platelet rich plasma (L-PRP), which has been recently used in dentistry and other medical fields to promote hard and soft tissue regeneration. AIM: The aim of this in vitro study was to evaluate the antimicrobial efficacy of lyophilized platelet lysate (LPL) against microorganisms like Enterococcus Faecalis, Bacillus Subtilis and Candida Albicans. MATERIALS AND METHOD: Discarded blood samples were obtained from blood banks after centrifugation, sterility check and activation. After this, lyophilization of the platelets was carried out. The antimicrobial activity of this freeze dried powder was evaluated by the minimum inhibitory concentration method and then by the Agar disk diffusion method. RESULTS: Lyophilized platelet lysate (LPL) inhibited the growth of Enterococcus faecalis, Bacillus subtilis and Candida albicans which is commonly associated with failed root canal treatment. CONCLUSION: LPL has the potential to be effective against the organisms which causes failure of root canal treatment. This can be of a valuable property in tissue engineering.