The Content of Arachidonic Acid in Menstrual Blood of Women with Genital Inflammatory Diseases
Journal: Ukrainian journal of medicine, biology and sport (Vol.3, No. 1)Publication Date: 2018-01-29
Authors : Varukha E. V. Mykhailychenko B. V.;
Page : 23-25
Keywords : forensic medicine; laboratory methods of research; regional origin of blood;
Abstract
The purpose of the study is to develop criteria for the establishment of blood menstrual origin with discovering the statistically significant amount of arachidonic acid in it. Materials and methods. The objects of the study were dried on gauze menstrual blood taken from practically healthy women and women who have genital inflammatory diseases aged from 18 to 45 with a normal menstrual cycle. It is generally known that menstrual blood, mixed with the contents of the vagina, contains both the components of the vaginal epithelium and the epithelium of the mucous membrane. Therefore, the presence of vaginal epithelium in blood is used to establish menstrual origin of blood. However, forensic medical practice shows that specific cells can be detected only in 50% of cases. In search of directions for the development of this problem, we drew attention primarily to biochemical studies, which are becoming increasingly used in forensic medicine, in particular on the biochemical properties of menstrual blood. It is known that menstrual blood has a specific odor, which is due to the presence of trimethylamine in it. In the physiological mechanisms of menstrual bleeding, biologically active substances are involved, in particular those derivatives of arachidonic acid, such as prostaglandins. The possibility of their use in forensic medical practice has not been developed. Therefore, the use of characteristic biochemical parameters and properties of menstrual blood can be used to determine its regional origin. This question is one of the complex and insufficiently developed in forensic medicine. The article provides information on the diagnostic capabilities of the detection of arachidonic acid in the blood of menstrual origin on physical evidence using the gas chromatography method. In forensic practice, this method is completely new. The obtained results allow it to be recommended for forensic purposes, particularly in investigation of crimes.
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