Dynamics of Laboratory Blood Indices in Rats after Hypodermic Implantation of a Composite Based on Polylactide, Hydroxyapatite and Tricalcium Phosphate

The article deals with the question of determining the dynamics of laboratory parameters of blood in rats after subcutaneous implantation of a composite based on polylactide, hydroxyapatite and tricalcium phosphate. The experiment was carried out on 15 white laboratory male rats, age of animals – 6 months, body weight – 240±25 g. We made a cut of the skin at the back of the rat laterally from the spine below the shoulder and input an implant (composite biomaterial) in the form of a disk (diameter – 5 mm, height – 2 mm) in the simulated "pocket" subcutaneous fat tissue of rats. The wound was sewn with silk. These animals' blood was taken after 30, 90 and 180 days after implantation. The control group of animals consisted of intact rats (n=5). We examined the blood of rats at 30, 90 and 180 days after implantation, the number of red blood cells, leukocytes and platelets, hemoglobin and leukogram. The following biochemical parameters were studied in blood serum of animals: glycoproteins, sialic acids, δ-glucosidates, activity of ALT and AST, acid and alkaline phosphatase, γ-glutamyltranspeptidase (GGT), bilirubin, urea and creatinine. During rheumatologic examination of rats at different observation periods (30, 90 and 180 days), no changes in the parameters of erythrocytopoiesis were established. Only at the 30th day of observation after implantation, there was an increase of 4.0 % relative number of band neutrophils, indicating the completion of inflammatory and regenerative processes in the subcutaneous tissue at the site of implantation. During the biochemical study of blood serum of rats, an increase in the content of glycoproteins was found to be 13.8 % compared to the control group. This indicates the completion of the regeneration process at the site of the implant, because later, the content of glycoproteins did not differ from the indicator in intact animals. When analyzing markers of the functional state of the liver and kidneys in animals subcutaneously implanted with composite material, their function impairments were not observed at all terms of the study. Thus, the introduction of implants based on polylactide, hydroxyapatite and tricalcium phosphate did not affect the somatic status of rats in experiments during its subcutaneous implantation in long-term observation. Lack of growth blood serum indicators of the functional state of the liver (ALT, AST, GGT) and the kidneys (creatinine, urea) at 30, 90 and 180 days proves the absence of nephrotoxicity and hepatotoxicity of the implanted implants.