EXTRACELLULAR BIOSYNTHESIS OF SILVER NANOPARTICLES USING FUNGUS PENICILLIUM SPECIES

Synthesis of nanoparticles by biological process is good alternative as compare to physical and chemical processes because it's very easy, non-toxic, eco-friendly, and cheap. Biological synthesis combines biological principles (i.e., reduction/oxidation) by microbial enzymes. The advantages of fungal-mediated biosynthesis of nanoparticles have turned the attention of scientists to the kingdom of fungi. The present research study shows the biological synthesis of silver nanoparticles (AgNPs) by using filamentous fungus Penicillium sp. The biomass was harvested after 72 hours of growth by sieving through a plastic sieve and from this biomass cell filtrate was obtained for the synthesis process. The synthesis process was quite fast; when silver ion was interact with cell filtrate, within few minutes. After 24 hour of incubation period in dark condition, colorless cell filtrate solution with AgNO3 solution was turns in to brown color solution; that means synthesis process of silver nanoparticles was confirmed due to color change reaction. The characterization of synthesized Silver nanoparticles was done by Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM), UV-Visible spectroscopy.