In vitro Propagation of Adenia hondala (Gaertn.) de Wilde

Adenia hondala (Gaertn.) de Wilde belonging to the family Passifloraceae is a perennial climbing herb with potential medicinal value. The possibility of in vitro clonal propagation of Adenia hondala was investigated by the use of nodal explants cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of BAP and KN. Optimum treatment was the combination of 1 mg.L-1 BAP and 0.5 mg.L-1 KN that enhanced percent response of explants and the number of multiple shoots per explants. An average of 10.23 shoots per explants was resulted after 32 days of culture. In vitro shoots were elongated in MS medium supplemented with 1 mg.L-1 KN. Half strength MS medium supplemented with 1 mg.L-1 IBA was found to be the best medium for rooting. The rooted plantlets were gradually acclimated ex vitro in mist chamber and successfully established under field conditions with high survival rate.