IN VITRO ANTI-UROLITHIATIC ACTIVITY OF FRESH JUICE OF CITRUS LIMON (L.)

The study was planned to investigate the anti-urolithiatic activity of fresh juice of Citrus limon L. fruit. The phytochemical screening was also undertaken to reveal the alkaloids, flavonoids, reducing sugar, vitamin C, amino acid and sterols present in the sample. Macromolecule supersaturation of higher molecular weight components of plant extract exerts its action similar to natural urinary inhibitors and inhibits crystal aggregation and growth. Pharmacological evaluation of Citrus limon L. was performed by the percentage dissolution and turbidity method. Calcium oxalate crystals were synthetically prepared and packed in a semi-permeable membrane in both methods. Result was compared statistically and it showed significant P<0.001 anti-urolithiasis activity as compared to control and standard (Cystone).