Influence of some phytohormones based culture medium on in-vitro multiplication of gerbera (Gerbera jamesonii)

The experiment was conducted during 2009-10 at Plant Tissue Culture Laboratory of Uttar Pradesh Horticulture Department, Lucknow and Bundelkhand University, Brahmanand Mahavidyalaya, Rath, U.P. to study the in vitro multiplication of gerbera cultivars (Tamara and Panama) from shoot bud and capitulum bud culture explants using MS medium supplemented with phytohormones i.e. BAP (0.5, 1, 2, 3 mg/l), Kinetin (0.5, 1, 2, 3 mg/l) and IAA (0.5, 1.0 mg/l). MS medium supplemented with BAP 2.0 mg/l + IAA 1.0 mg/l gave the highest culture regeneration, number of multiple shoots, number of leaves per plant, shoot length and took least days to bud break in both-shoot bud and capitulum bud culture taken as explant.