Optimization of Emulgel Formulation of The Defatted Ethyl Acetate Extract of Mychorriza arbuscule Induced Ginger Rhizomes (Zingiber officinale Rosc.)

The research aim was to develop an emulgel formulation of the defatted ethyl acetate extract of Mychorriza arbuscule fungi induced ginger rhizomes (MIG) as a topical anti-inflammation dosage form by using optimization technique. A complete randomized design with two factors and two levels was used. The concentration of gelling base (hydroxyl propyl methyl cellulose, HPMC, at levels 0.75 and 2%) and the concentration of the extract (at levels 8 and 16%) were used as independent variables while the viscosity and the percentage of edema inhibition of the dosage form were taken as the response variables. Four (2 2) formulations were prepared: F1, Fa, Fb, and Fab. As a marker, 6-gingerol was used which concentration was determined by TLC-densitometry with silica gel GF 254 as a stationary phase and n-hexane : ethyl acetate (13:7) as a mobile phase. Data were analyzed using multi regression analysis, the optimum formula was obtained from the intercept of the superimposed contour plot. The TLC densitometry system showed that the marker and the extract had a retention factor of 0.3. The 6-gingerol concentration of F1 was 0.31%; Fa 0.38%; Fb 0.82%; Fab 0.81%; and the defatted extract 3.67%. The viscosity of each formula was 10 (F1), 200 (Fa). 5 (Fb) and 150 (Fab) dPa.s, respectively. The percentage of edema inhibition of the dosage form was 31.6% (F1), 31.3% (Fa), 45.5% (Fb) and 43.7% (Fab), respectively. The optimum formula of the emulgel is at HPMC concentration of 2% and the extract 12% which has a viscosity of 127.129 dPa.s and edema inhibition 37.8%.