PHYTOCHEMICAL ANALYSIS, CHEMICAL CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF HELICANTHUS ELASTICA DESR

The objective of present studies deals with the Phytochemical analsis, chemical characterization and antioxidant activity of Helicanthus elastica Desr. Total phenolic content, Total flavonoid content, Total proanthocyanidin content of ethyl acetate fraction was determined. Antioxidant activity was carried out by Nitric oxide scavanging activity, DPPH scavenging activity, reducing power assay and anti-lipid peroxidation activity. Characterization of ethyl acetate fraction of H.elastica carried out by LC-MS/MS method. The HPTLC together with LC-MS/MS study confirm the presence of quercetin and epicatechin in ethyl acetate extract. Quantitative studies reveal that plant contains higher amount of phenolics such as flavonoids and proanthocynidins. Antioxidant activity in various in vitro models demonstrated that extract have significant radical scavenging as well as antioxidant activity which is consistent with its higher flavonoid content. Key Words: Helicanthus elastica, ethyl acetate fraction, characterization, flavanoid, antioxidant