Preparation of nanohybrid antibiotic from levofloxacin and determination its inhibitory efficacy against Staphylococcus aureus isolated from diabetic foot ulcer

Objective: This study aimed to Prepare nano hybrid from levofloxacin antibiotic and evaluation of its inhibitory effect against Staphylococcus aureus from diabetic foot ulcer. Methods: A total 43 swab samples were collected from diabetic foot ulcer patients hospitalized in AL-Hussein Teaching Hospital in Sacred Kerbala province, it has been obtained 9 isolates were Staphylococcus aureus. A nanohybrid antibiotic LEV-LDH was prepared using direct ion exchange between antibiotic levofloxacin (LEV) and layered double hydroxide (LDH). The new nano antibiotic was identified. FT-IR spectroscopy revealed the appearance of new groups in the LEV-LDH spectrum, which indicates the formation of a new compound. The X-Ray Diffraction (XRD) spectrum revealed the appearance of new diffraction planes in the nanohybrid antibiotic spectrum in compare with spectrum of layered double hydroxide. The two and three-dimensional image of the above compound in atomic force microscope confirmed the formation of new antibiotic with nano dimensions. The antimicrobial activity of the nanohybrid antibiotic was studied against 9 isolates of S. aureus. Results: The results showed that the highest inhibition was obtained against isolate 3 with average inhibition zone of 34.642 mm. The combination between the antibiotics LEV-LDH and teicoplanin was studied against S. aureus bacteria, the results showed a synergistic inhibition effect against some isolates while others gave antagonist inhibition effect. Conclusion: The efficacy of the nanohybrid levofloxacin antibiotic in inhibition of isolated S. aureus.