Importance of homogenization in platelet-rich plasma researches

Aim: To achieve homogenization, one of the most important factors to overcome during platelet-rich plasma (PRP) is standardization phases. Method: We used 10 cc 'BD vacutainer ACD-A' tubes which consisted of 1.5 cc ACD-A. 3 tubes blood was collected from healthy volunteers from 20 to 40 years. All tubes were centrifuged by 180g x 12 min. Then 0.01 ml PRP was collected from just the level of buffy coat. Results: Platelet concentration decreased from buffy coat to top of PPP as expected. If any counting done before homogenization the results will be misleading. Conclusion: When we sample 3 cc PRP homogenized with shaker at 3 point from 3 different point, we see that they are close to each other. It is important that the PRP to be used in the studies must be homogenized before any measurement.