THE ACTIVITY OF MONOCYTES/MACROPHAGES IN PATIENTS WITH ISCHEMIC CHRONIC HEART FAILURE

Chronic heart failure (CHF) is a complex clinical syndrome that results from any structural or functional impairment of ventricular filling or ejection of blood. It is a major health issue in society today, because it is associated with health, death and consumption of healthcare resources. HF affects approximately 1-2% of adults in developed countries, and prevalence increases markedly with age: 10% in cohort 75 years and older. The aim of study was to evaluate of functional activity of blood monocytes/macrophages in patients with ischemic CHF by identification of their spontaneous production of cytokines. Material and methods. 96 patients with CHF of ischemic genesis were observed. The diagnosis was verified by clinical, laboratory and instrumental methods according to European Society of Cardiology recommendations (2013, 2016). Patients were divided into 3 subgroups (according New-York Heart Association (NYHA) functional class (FC) classification of HF): FC II (NYHA) – 27 patients, FC III (NYHA) – 39 patients and FC IV (NYHA) – 30 patients. Control group consist of 30 practically healthy persons. Suspension of monocytes from blood obtained by Recalde H. method. Interleukin 1b (IL-1b), interleukin 6 (IL-6), and interleukin 10 (IL-10 ) levels in culture supernatant were determined using commercial ELISA kits (ProCon, Russia; Amersham Pharmacia Biotech, UK) according to the manufacturer's instructions. Statistical analyses were performed using the Statistica 12.0 (StatSoft, Tulsa, OK, USA). Statistical significance was assumed at p<0.05. Results. The in vitro spontaneous production of proinflammatory cytokines IL-1b and IL-6 by monocytes/macrophages in patients with HF was significant higher than in control group: (119.50±4.12) pg/106 cells vs (51.39±3.71) pg/106 cells (p<0.001) and (6.62±0.41) pg/106 cells vs (2.79±0.28) pg/106 cells (p<0.001) respectively. Instead the spontaneous production of anti-inflammatory IL-10 was lower in HF: (3.79±0.41) pg/106 cells vs (5.86±0.76) pg/106 cells (p<0.01). The severity of HF was associated with increased production by monocytes/macrophages of IL-1b and IL-6 and with decreased production of IL-10. Conclusion. The monocytes/macrophages in patients with ischemic HF are in condition of chronic activation which manifests of overproduction of proinflammatory cytokines and poor secretion of anti-inflammatory IL-10.