The Virtual Matrix Based Methods with Method Based on the Support Vector Machines and Artificial Neuronal Network

Propred ?I and Propred are the matrix based method while CTL Pred is a method based on the combined approach of support vector machine and artificial neuronal network. Promiscuous T-cell epitopes make ideal targets for vaccine development. PfM18AAP is a malarial antigenic protein responsible for rupturing of RBC cells. The PfM18AAP of Plasmodium falciparum malaria is a metallo-aminopeptidase that shows highly restricted specificity for peptides with an N-terminal Glu or Asp residue. Thus, it is responsible in effecting the complete degradation or turnover of proteins, such as host hemoglobin, which provides a free amino acid pool for the growing parasite Thus, used method allows us identification of MHC class I binders (peptides binding with many MHC alleles) & MHC-II binders having proteasomal cleavage site at C-terminus. The user-friendly result display format (HTML-II) can assist in locating the promiscuous MHC binding regions from antigen sequence.