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Using Hydrogen Peroxide for Reducing Bacterial Contamination in Date Palm Tissue Culture

Journal: International Journal of Advances in Agricultural Science and Technology (IJAAST) (Vol.5, No. 4)

Publication Date:

Authors : ; ; ;

Page : 25-33

Keywords : Date palm; tissue culture; bacterial contamination; hydrogen peroxide; decontaminating agents;

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The use of hydrogen peroxide as a disinfectant material was evaluated for its effect relevant to bacterial contamination which usually occurs in the micropropagation of date palm. Different concentrations of hydrogen peroxide at different time intervals were examined and compared to ethyl alcohol and distilled water with both of callus and shoot explants of date palm cv. Sewi. The most powerful disinfection with 100% survival occurred by using 10 ppm hydrogen peroxide for 1 min.

Last modified: 2018-04-15 16:07:29