SPECTRUM OF MICROORGANISMS ISOLATED FROM BLOOD CULTURE AND THEIR RESISTANCE PATTERN

The aim of the present study was to determine the spectrum of microorganisms isolated from blood culture and their resistance pattern.This study was carried out between January 2014 and December 2014 in the Department of Microbiology , Era's Lucknow Medical college & Hospital, Lucknow . A total of 2278 blood culture samples of patients with suspected sepsis were included in the study. BHI broth and BACTEC aerobic & anaerobic culture bottles were used for taking cultures.All bottles were subcultured onto Blood and MacConkey's agar. In case of any growth , it was identified and antibiotic susceptibility test was done according to standard procedures.Cultures were positive in 278 samples (12.2%). Spectrum revealed that Coagulase Negative Staphylococci accounted for 34.89%,Staphylococcus aureus17.98% and Klebsiella spp9.35% . Staphylococcus strains showed Methicillin resistance in 10% of isolates. Extended spectrum beta lactamases constituted 26.92% each for Klebsiella and Escherichia coli strains and 40% of Citrobacter strains isolated .Vancomycin resistant Enterococcus strains accounted for 10% of strains isolated .A large proportion of patients presenting with sepsis at a tertiary care hospital, are already treated with antibiotics elsewhere. This leads to a low positive yield in blood culture.The present study highlights the increasing resistance in microorganisms causing sepsis Their early detection and resistance pattern will definitely help in modifying the treatments. Thus early blood culture report increases therapeutic compliance.