DEVELOPMENT OF LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS DETERMINATION OF BROMHEXINE HYDROCHLORIDE AND ENROFLOXACIN IN FORMULATIONS
Journal: Indian Drugs (Vol.55, No. 02)Publication Date: 2018-02-26
Authors : Choksi P. a Shaikh F. a Shah D. A. a Agarwal K. a Mehta F. A. a; Patel V. B. a;
Page : 44-49
Keywords : ;
Abstract
A simple, specific, accurate, precise and reproducible method has been developed and validated for the estimation of bromhexine hydrochloride and enrofloxacin in fixed dose combination using RP-HPLC. The separation was achieved using stationary phase ODS Hypersil C18 column (250 mm× 4.6 mm i.d.) in isocratic mode, with mobile phase containing 0.05 M potassium dihydrogen phosphate buffer (pH 4 by o-phosphoric acid) : methanol: acetonitrile : triethylamine (40:20:40:01), at a flow rate of 1.0mL/min and eluents were monitored at 256 nm. The retention time of enrofloxacin and bromhexine HCl were found to be 3.00 min and 5.1 min respectively. The linearity for bromhexine HCl and enrofloxacin was in the range of 2-15 μg/mL and 20-150 μg/mL, respectively. The method was validated as per ICH guideline. The recoveries of bromhexine HCl and enrofloxacin were found in the range of 99.61-101.65% and 99.52-100.13 %, respectively. The method was successfully applied for the determination of both the drugs in combined dosage form.
Other Latest Articles
- Solitary radiolucency near the angle of mandible
- ENANTIOSELECTIVE ANALYSIS OF GUAIFENESIN IN BULK AND PHARMACEUTICAL DOSAGE FORMS BY CHIRAL REVERSE PHASE HPLC-PDA METHOD
- Incidence of mandibular nutrient canals in a hypertensive and diabetic population: A prospective cross-sectional radiographic study
- Age estimation from pulp/tooth area ratio by panoramic radiographs: In Kashmiri population
- FORMULATION AND EVALUATION OF NATEGLINIDE NANOSPONGES
Last modified: 2018-04-19 20:53:14