FORMULATION AND EVALUATION OF CHITOSAN NANOPARTICLE LOADED WITH PHYLLANTHIN

Objective: The aim of the work was to formulate and evaluate chitosan nanoparticle loaded with Phyllanthin. Methods: Chitosan nanoparticle loaded with phyllanthin were prepared by ionic chelation method using chitosan solution, prepared by dissolving chitosan flakes in 1.5%glacial acetic acid to this STPP (0.10 to 0.60 %W/V),phyllanthin and was added to chitosan solution. The prepared chitosan nanoparticle loaded with phyllanthin from all batches (F1-F6) were characterized for its morphology, particle size, drug loading capacity ,entrapment efficiency, invitro release study ,stability study. The chitosan nanoparticle have an average particle size of 1.0 ± 5.0µm, The % entrapment efficiency of phyllanthin was found to be 34.4-74.5%w/w. The % drug loading capacity was 34-60.5%w/w. The drug content in chitosan nanoparticle was found to be 69 -80.5%w/w. The invitro release studies of all the formulations F1-F6 was carried out and result have shows that be F6 were most stable formulation and good release. Conclusion: The evaluation results have shown that formulation F6 was found to be more stable with good release charateristics invitro and have good bioavailability. Keywords: Nanoparticle,Phyllanthus niruri,Chitosan,Ionic chelation