SYNTHESIS OF NEW PYRIMIDINE DERIVATIVES AS POTENTIAL ANTICANCER AGENT

We have tried to synthesize a series of 5 derivatives of Pyrimidine. Synthesis was carried out according to reactions shown in Reaction Scheme. At first, 4th position of 2,4 dichloropyrimidne was substituted by Anthranilic acid to form Synthesis of 2-(2- chloropyrimidin-4-ylamino)benzoic acid 1[C]. 5amino-1,3,4-thiadiazole 2[B] was prepared using Synthesis of 2-(2-chloropyrimidin-4ylamino)benzoic acid 1[C] and Thiosemicarbazide as starting material. Further confirmation was carried out by IR which showed the presence of amino (-NH2) band ~3422.80 cm-1, 1H NMR spectra which revealed all the corresponding peaks at δ=4-8 ppm for aromatic protons. MASS spectrum showed M+1 peak at 305.4 Various Substituted Pyrimidine derivatives (3[B-1]-3[B-5]) were prepared from substituted aniline(3[A-1]-3[A-5]) by reacting with5-[2-((2-chloropyrimidine- 4yl)amino)phenyl)-1,3,4-thiadiazol-2-amine 2[B] .The reaction was monitored by Thin-layer chromatography using suitable mobile phase such as Chloroform: Methanol (9:1); nhaxane:ethyl acetate (5:5). The Rf values were compared and found that they were different from each others. The melting point of the derivatives was determined. Spectral study of all the derivatives of substituted Pyrimidine derivatives was carried out using IR, 1H NMR, and MASS which leads us to believe that all the derivatives has been properly synthesized. Key Words: Anticancer activity, synthesis, characterisation.