Phenolics, Flavonoids, and Antioxidant Activity of Alstonia scholaris R.Br Stem Bark Extract

Alstonia scholaris belongs to family A as t R.Br pocynaceae, is one of medicinal forest plant raditional medicine to treat fever, malaria, cough with phlegm, diarrhea, diabetes, cholesterol-lowering, intestinal worms, acute rheumatism, ulcers, and hypertension. One of the causes of heart disease, atherosclerosis, and cancer is oxidative stress. The stress can be cured or reduced by taking a plant ntioxidant. Flavonoid, a phenol compound class, is one of the secondary metabolites that function as antioxidant. This determines total levels of phenol, flavonoid, and antioxidant activity of stem paper Alstonia bark extract ( R. Br). with the folin-ciocalteu method Alstonia scholaris Quantitative determination of total phenolics expressed as mg of gallic acid equivalent (GAE) per gram of extract total flavonoids 3 , content by AlCl method expressed as Quercetin equivalen (QE) with (2,2-diphenyl-1-picrylhydrazyl) method , and in vitro antioxidant activities DPPH expressed in terms IC (inhibition concentration). esult showthat extraction of three replicates in maceration with of R s 50 96% ethanol yielded 4.19 % . The total phenolic content was mg GAE/g extract, while the total flavonoid filtrate 51.50 content was 0.35 mg QE/g extract. antioxidant activity of stem bark extract was 211.54 g/mL. IC value of assay μ 50